Bacterial flora as indicated by PCR-temperature gradient gel electrophoresis (TGGE) of 16S rDNA gene fragments from isolated guts of phlebotomine sand flies (Diptera: Psychodidae)
نویسندگان
چکیده
منابع مشابه
New Records of Phlebotomine Sand Flies (diptera: Psychodidae) from Ecuador.
The number of recorded phlebotomine sand fly species in Ecuador has nearly doubled during the past 20 years as a result of surveys. In 2005, a sand fly survey of two localities, Tiputini in the Amazon rain forest and Paraiso Escondido in the Pacific coastal lowland forest, resulted in the capture of 25 species. New records for Ecuador consisted of five species from the Amazonian region and one ...
متن کاملAnthropophilic and zoophilic phlebotomine sand flies (Diptera, Psychodidae) from Thailand.
As a result of extensive surveys of sand flies carried out during 1987-90 in Thailand, species including a man-biter, Phlebotomus hoepplii, and cattle-biters, P. argentipes and P. major major, were discovered in Kanchanaburi Province. The human-baited catches revealed a low density of P. hoepplii and the biting occurred during 2100-2300 hours. Phlebotomus hoepplii was also found feeding on buff...
متن کاملSampling strategies for phlebotomine sand flies (Diptera: Psychodidae) in Europe.
The distribution of phlebotomine sand flies is widely reported to be changing in Europe. This can be attributed to either the discovery of sand flies in areas where they were previously overlooked (generally following an outbreak of leishmaniasis or other sand fly-related disease) or to true expansion of their range as a result of climatic or environmental changes. Routine surveillance for phle...
متن کاملCharacterization of wine yeasts by temperature gradient gel electrophoresis (TGGE).
18S rDNA from 74 wine yeast strains was amplified by PCR using specific primers, and the products analyzed by temperature gradient gel electrophoresis (TGGE). TGGE is a useful method in screening the genotypes of the wine yeasts. Intraspecific differentiation was achieved on the basis of TGGE in some cases, whereas in others identical bands for strains classified as separate species were obtain...
متن کاملQuantitative PCR: the determination of template copy numbers by temperature gradient gel electrophoresis (TGGE).
Quantitative PCR: the determination of template copy numbers by temperature gradient gel electrophoresis (TGGE) Low copy numbers of DNA or RNA sequences can be quantified easily by combining PCR (Polymerase Chain Reaction) and TGGE (Temperature Gradient Gel Electrophoresis). This combination allows to overcome the problems of quantitive PCR caused by plateau effects, uneven priming or, variable...
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ژورنال
عنوان ژورنال: Journal of Vector Ecology
سال: 2011
ISSN: 1081-1710
DOI: 10.1111/j.1948-7134.2011.00124.x